研究生教育

        致力于利用高通量测序，以CRISPR介导的基因编辑和表观遗传编辑为主要技术手段，结合基因组学、表观遗传学、信息学、基因编辑技术和动物体内模型，研究乳腺干细胞的维持分化及乳腺发育及乳腺癌的分子机制。

学历学位

2003~2008   博士，上海生化细胞所，中国科学院导师：胡赓熙研究员

1999~2003   学士，生命科学院，中国科学技术大学

工作经历

2018~至今   研究员/长聘轨助理教授，浙江大学爱丁堡联合学院

2015~2018   Staff scientist，美国国立卫生研究院

2014~2015   博士后，美国国立卫生研究院

导师：Dr. Keji Zhao

2009~2014   博士后，美国国立卫生研究院

导师：Dr. Ke Gai

主持基金

国家自然科学基金面上项目 2020-2024

荣誉奖励

2019       浙江省嘉兴市“创新嘉兴精英引领计划”创新人才

2011       美国国立卫生研究院优秀科研奖（Fellows Award for Research Excellence）

2004       中国科学院优秀学生奖

1999~2003  中国科学技术大学优秀学生奖一等奖

学术论文

一作及通讯作者论文

1.Zeng X*, Lee HK*, Wang C*, Achikeh P, Chengyu Liu, Hennighausen L. Interdependence of mammary-specific super-enhancer and its native promoter facilitates gene activation during pregnancy. Exp Mol Med.2020 Accepted. (* equal contribution)

2.Shin HY*, Wang C*, Lee HK*, Yoo KH, Zeng X, Kuhn T, Yang CM, Mohr T, Liu C, Hennighausen L. CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome. Nat Commun. 2017 May 31;8:15464. (* equal contribution)

3.Zeng X, Willi M, Shin HY, Hennighausen L* and Wang C*.Lineage-specific and non-specific cytokine-sensing genes respond differentially to the master regulator STAT5. Cell Rep 2016 Dec 20;17(12):3333-3346. (* Corresponding author)

4.Wang C, Xu S, Lee JE, Cho YW, Macfarlan T, Cui K, Liu C, Peng W and Ge K. Enhancer priming by H3K4 methyltransferase MLL4 controls cell fate transition. Proc Natl Acad Sci U S A. 2016 Oct 18;113(42):11871-11876. Recommended article in F1000Prime

5.Lee JE*, Wang C*, Xu S, Cho YW, Wang L, Feng X, Baldridge A, Sartorelli V, Zhuang L, Peng W, Ge K. H3K4 mono- and di-methyltransferase MLL4 is required for enhancer activation during cell differentiation.Elife. 2013 Dec 24;2(0):e01503. (* equal contribution)  Recommended article in F1000Prime

6.Wang C, Lee JE, Cho YW, Xiao Y, Jin Q, Liu C, Ge K. UTX regulates mesoderm differentiation of embryonic stem cells independent of H3K27 demethylase activity. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15324-9.

7.Hu Z*, Wang C*, Xiao Y, Sheng N, Chen Y, Xu Y, Zhang L, Mo W, Jing N, Hu G. NDST1-dependent heparan sulfate regulates BMP signaling and internalization in lung development. J Cell Sci. 2009 Apr 15;122(Pt 8):1145-54. (* equal contribution).

8.Wang C, Xiao Y, Hu Z, Chen Y, Liu N, Hu G. PEG10 directly regulated by E2Fs might have a role in the development of hepatocellular carcinoma. FEBS Lett.2008 Aug; 582(18):2793-8.

参与作者论文

1.Jang Y, Broun A, Wang C, Park YK, Zhuang L, Lee JE, Froimchuk E, Liu C, Ge K. H3.3K4M destabilizes enhancer H3K4 methyltransferases MLL3/MLL4 and impairs adipose tissue development. Nucleic Acids Res. 2018 Oct 18.

2.Willi M, Smith HE, Wang C, Liu C, Hennighausen L. Mutation frequency is not increased in CRISPR-Cas9-edited mice. Nat Methods. 2018 Oct;15(10):756-758.

3.Seok S, Kim YC, Byun S, Choi S, Xiao Z, Iwamori N, Zhang Y, Wang C, Ma J, Ge K, Kemper B, Kemper JK. Fasting-induced JMJD3 histone demethylase epigenetically activates mitochondrial fatty acid b-oxidation. J Clin Invest. 2018 Jul 2;128(7):3144-3159 

4.Yan J, Chen SA, Local A, Liu T, Qiu Y, Dorighi KM, Preissl S, Rivera CM, Wang C, Ye Z, Ge K, Hu M, Wysocka J, Ren B. Histone H3 Lysine 4 monomethylation modulates long-range chromatin interactions at enhancers. Cell Res. 2018 Jan 9. Doi:10.1038/cr.2018.1.

5.Local A, Huang H, Albuquerque CP, Singh N, Lee AY, Wang W, Wang C, Hsia JE, Shiau AK, Ge K, Corbett K, Wang D, Zhou H, Ren B. Identification of H3K4me1-associated proteins at mammalian enhancers. Nat Genet. 2018 Jan;50(1):73-82.

6.Shpargel KB, Starmer J, Wang C, Ge K, Magnuson T. UTX-guided neural crest function underlies craniofacial features of Kabuki syndrome.Proc Natl Acad Sci U S A. 2017 Oct 24;114(43):E9046-E9055.

7.Placek K, Hu G, Cui K, Zhang D, Ding Y, Lee JE, Jang Y, Wang C, Konkel JE, Song J, Liu C, Ge K, Chen W, Zhao K.  MLL4 prepares the enhancer landscape for FoxP3 induction via chromatin looping. Nat Immunol. 2017 Sep;18(9):1035-1045.

8.Willi M, Yoo KH, Reinisch F, Kuhns TM, Lee HK, Wang C, Hennighausen L. Facultative CTCF sites moderate mammary super-enhancer activity and regulate juxtaposed gene in non-mammary cells. Nat Commun. 2017 Jul 17;8:16069.

9.H3K4 methyltransferase activity is required for MLL4 protein stability. Jang Y, Wang C, Zhuang L, Liu C, Ge K. J Mol Biol. 2017 Jun 30;429(13):2046-2054.

10.Lee HK, Willi M, Wang C, Yang CM, Smith HE, Liu C, Hennighausen L. Functional assessment of CTCF sites at cytokine-sensing mammary enhancers using CRISPR/Cas9 gene editing in mice. Nucleic Acids Res. 2017 May 5;45(8):4606-4618

11.Willi M, Yoo KH, Wang C, Trajanoski Z, Hennighausen L. Differential cytokine sensitivities of STAT5-dependent enhancers rely on Stat5 autoregulation. Nucleic Acids Res. 2016 Dec 1;44(21):10277-10291.

12.Shin HY, Willi M, Yoo HK, Zeng X, Wang C, Metser G, Hennighausen L.Hierarchy within mammary STAT5-driven Wap super-enhancer. Nat Genet. 2016 Aug;18(8):904-11.

13.Xiao Y, Thoresen DT, Miao L, Williams JS, Wang C, Atit RP, Wong SY, Brownell I. A cascade of Wnt, Eda, and Shh signaling is essential for touch dome merkel cell development. PLos Genet. 2016 Jul 14;12(7):e1006150.

14.Faralli H, Wang C, Nakka K, Benyoucef A, Sebastian S, Zhuang L, Chu A, Palii CG, Liu C, Camellato B, Brand M, Ge K, Dilworth FJ. UTX demethylase activity is required for satellite cell-mediated muscle regeneration. J Clin Invest. 2016 Apr 1;126(4):1555-65

15.Benyoucef A, Palii CG, Wang C, Porter CJ, Chu A, Dai F, Tremblay V, Rakopoulos P, Singh K, Huang S, Pflumio F, Hébert J, Couture JF, Perkins TJ, Ge K, Dilworth FJ, Brand M. UTX inhibition as selective epigenetic therapy against TAL1-driven T-cell acute lymphoblastic leukemia. Genes Dev. 2016 Mar 1;30(5);508-21.

16.Yoo KH, Oh S, Kang K, Yang CM, Wang C, Ge K, Robinson GW, Hennighausen L.Histone demethylase KDM6A controls the mammary luminal lineage through enzyme-independent mechanisms. Molecular and Cellular Biology. 2016 Jul 29;36(16):2108-20.

17.Metser G, Shin HY, Wang C, Yoo KH, Oh S, Villarino AV, O'Shea JJ, Kang K, Hennighausen L. An autoregulatory enhancer controls mammary-specific STAT5 functions. Nucleic Acids Res. 2016 Feb 18;44(3):1052-63.

18.Xiao Y, Thoresen DT, Williams JS, Wang C, Perna J, Petrova R, Brownell I. Neural Hedgehog signaling maintains stem cell renewal in the sensory touch dome epithelium. Proc Natl Acad Sci U S A. 2015 Jun 9;112(23):7195-200.

19.Shi X, Zhang Z, Zhan X, Cao M, Satoh T, Akira S, Shpargel K, Magnuson T, Li Q, Wang R, Wang C, Ge K, Wu J.An epigenetic switch induced by Shh signalling regulates gene activation during development and medulloblastoma growth. Nat Commun. 2014 Nov 5;5:5425.

20.Jin Q, Wang C, Kuang X, Feng X, Sartorelli V, Ying H, Ge K and Dent S. Gcn5 and PCAF regulate PPARγ and Prdm16 expression to facilitate brown adipogenesis. Molecular and Cellular Biology. 2014 Oct 1;34(19):3746-53.

21.Jin Q, Zhuang L, Wang C, Dolan B, Wang Z, Lu Y, Lai B, Zhao K, Peng W, Dent S and Ge K.Gcn5 negatively regulates interferon β production in cells. EMBO Reports. 2014 Nov;15(11):1192-201.

22.Wang AH, Zare H, Mousavi K, Wang C, Moravec CE, Sirotkin HI, Ge K, Gutierrez-Cruz G, Sartorelli V.The histone chaperone Spt6 coordinates histone H3K27 demethylation and myogenesis. EMBO J. 2013 Apr 17;32(8):1075-86.

23.Hu G, Cui K, Northrup D, Liu C, Wang C, Tang Q, Ge K, Levens D, Crane-Robinson C, Zhao K. H2A.Z Facilitates Access of Active and Repressive Complexes to Chromatin in Embryonic Stem Cell Self-Renewal and Differentiation. Cell Stem Cell. 2013 Feb 7;12(2):180-92.

24.Jin Q, Yu LR, Wang L, Zhang Z, Kasper LH, Lee JE, Wang C, Brindle PK, Dent SY, Ge K. Distinct roles of GCN5/PCAF-mediated H3K9ac and CBP/p300-mediated H3K18/27ac in nuclear receptor transactivation. EMBO J. 2011 Jan 19;30(2):249-62.

25.Xiao Y, Ge M,Xue X, Wang H, Wang CC, Wu XF, Li L, Liu LL, Qi XM, Zhang YH, Li Y, Xie TP, Gu J, Ren J. Hepatic cytochrome P450s metabolize aristolochic acid and reduce its kidney toxicity. Kidney Int. 2008 Jun; 73(11):1231-9.

26.Chen Y, Zhou X, Liu N, Wang C, Zhang L, Mo W, Hu G. Arginine methylation of hnRNP K enhances p53 transcriptional activity. FEBS Lett. 2008 May; 582(12):1761-5.