研究生教育

胡汛，男，教授，博士生导师，浙江大学求是特聘教授，1988-1990年曾于德国Wurzburg 大学，生物技术中心担任博士后，1990-1991年于美国Mount Sinai Medical School生理系担任博士后，现任浙江省医学分子生物学重点实验室主任，浙江大学恶性肿瘤预警与干预教育部重点实验室主任，浙江大学肿瘤研究所常务副所长。

科研成果

曾获1998年度美国癌症学会- Pharmacia Upjohn 年轻研究者奖。近五年在多环芳烃与肿瘤研究中的成果已整理成30篇论文，分别发表在国际著名学术刊物。他的论文被美国、德国、英国、法国等欧美科学工作者应用300余次。他和研究团队一起发现了“饿死”癌细胞的新疗法，并发表在国际生物和医学领域权威杂志elife上，得到了国际著名肿瘤学者的肯定。

学术任职

1991-95副教授，浙江医科大学肿瘤研究所。

1995-97Research associate, 美国Pittsburgh Mercy Hospital, 肿瘤研究室

1997-99Research scientist, Pittsburgh Mercy Hospital, 肿瘤研究室

1999-00研究员, Tissue Transformation Techologies, Edison, NJ, USA

2000-01资深研究员, Wyeth Ayerst（惠氏制药）新药研发部, Princeton, NJ, USA.

2001-06教授, (长江教授), 浙江大学医学院肿瘤研究所。

2006-教授, (求是教授), 浙江大学医学院肿瘤研究所。

2003-副所长, 浙江大学医学院肿瘤研究所.

2003-主任，浙江省医学分子生物学重点实验室.

2010-  主任，浙江大学教育部恶性肿瘤预警与干预重点实验室.

代表性论文

1.CMJin, XB Zhu, H Wu, Y Wang, X Hu*. Perturbation of phosphoglycerate kinanse 1 (PGK1) only marginally affects glycolysis in cancer cells. J Biol Chem. (2020) 295 6425-6446.

2.MF Ying, C Guo, X Hu*. The quantitative relationship between isotopic and net contributions of lactate and glucose to the tricarboxylic acid (TCA) cycle. J Biol Chem. (2019) 294:9615-9630

3.W Zhang, C Guo, K Jiang, M Ying, X Hu* Quantification of lactate from various metabolic pathways and quantification issues of lactate isotopologues and isotopmers. Scientific Reports| 7: 8489 | DOI:10.1038/s41598-017-08277-3

4.J Xie, C Dai, X Hu* Evidence That Does Not Support Pyruvate Kinase M2 (PKM2)-catalyzed Reaction as a Rate-limiting Step in Cancer Cell Glycolysis. J Biol Chem (2016) 291:8987-8999

5.M Chao, A nonrandomized cohort and a randomized study of local control of large hepatocarcinoma by targeting intratumoral lactic acidosis eLife eLife 2016;5:e15691. DOI: 10.7554/eLife.15691

6.J Xie, H Wu, C Dai, Q Pan, Z Ding, D Hu, B Ji, Y Luo*, X Hu*. Beyond Warburg effect – dual metabolic nature of cancer cells Scientific Reports | 4 : 4927 | DOI: 10.1038/srep04927

7.Zhang B, Liu Z, Hu X* . Inhibiting cancer metastasis via targeting NADPH oxidase 4. Biochem Pharmacol. 86:253-266, 2013

8.Wu H, Ding Z, Hu D, Sun F, Dai C, Xie J, Hu X* Central role of lactic acidosis in cancer cell resistance to glucose deprivation-induced cancer cell death. J Pathol. 227:189-199, 2012

9.Chen J, Xie J, Jiang Z, Wang B, Wang Y, Hu X*.Shikonin and its analogs inhibit cancer cell glycolysis by targeting tumor pyruvate kinase M2. Oncogene 30:4297:4306, 2011

10.Xuan Y, Hu X*. Naturally-occurring shikonin analogues- a class of necroptotic inducers that circumvent cancer drug resistance. Cancer lett 274-233-242, 2009

11.Han W，Xie J, Li L, Liu Z, Hu X* Necrostatin-1 reverts shikonin-induced necroptosis to apoptosis. Apoptosis 14:674-686, 2009

12.Lu Y, Xu X, Din Z, Liu Z, Zhang B, Yan Z, Sun J, Hu S*, Hu X* Complete  genome  of Phenylobacterium  zucineum  -  a  novel  facultative  intracellular bacterium isolated from human erythroleukemia cell line K562.BMC Genomics9:368, 2008, e-pub ahead of print.

13.Hu X*, Xuan Y. Bypassing Cancer Drug resistance by activation of multiple death pathways – a proposal from the study circumventing cancer drug resistance byinduction of necroptosis. Cancer Lett.259：127-137, 2008

14.Li L, Pan Q, Han W, Liu Z, Li L, Hu X*.Schisandrin B Prevents Doxorubicin-Induced Cardiotoxicity via Enhancing Glutathione Redox Cycling.Clin Cancer Res. 2007, 13(22):6753-60  

15.Hu X*, Han W, Li L.Targeting the weak point of cancer by induction of necroptosis.Autophagy. 2007,3(5):490-2.

16.Han W, Li L, Qiu S, Lu Q, Pan Q, Gu Y, Luo J, Hu X*. Shikonin circumvents cancer drug resistance by induction of a necroptotic death. Mol Cancer Ther. 2007, 6(5):1641-9

17.Li L, Han W, Gu Y, Qiu S, Lu Q, Jin J, Luo J,Hu X*. Honokiol induces a necrotic cell death through the mitochondrial permeability transition pore. Cancer Res. 2007, 67(10):4894-903.

18.Zhang K, Han W, Zhang R, Xu X, Pan Q, Hu X*.Phenylobacterium zucineum sp. nov., a facultative intracellular bacterium isolated from a human erythroleukemia cell line K562. Syst Appl Microbiol. 2007, 30(3):207-12.

19.Pan Q, Lu Q, Zhang K, Hu X*.  Dibenzocyclooctadiene – a class of novel inhibitors of P-glycoproteins. Cancer Chemother Pharmacol 58:99-106, 2006.

20.Li L, Pan Q, Sun M, Lu Q,Hu X* Dibenzocyclooctadiene-a class of novel inhibitors of multidrug resistance. Life Sciences. 80：741-748，2007。

21.Li L, Lu Q, Hu X*. Schisandrin B enhances doxorubicin-induced apoptosis of cancer cells but not normal cells. Biochem Pharmcol71:584-595, 2006.

22.Pan Q, Wang T, Lu Q, Hu X*. Schisandrin B – a novel inhibitor of P-glycoprotein.  Biochem Biophys Res Comm.  335:406-411, 2005.

23.Sun M,  Xu X,  Lu Q,  Pan Q, Hu X*. Schisandrin B –a Dual Inhibitor of P-glycoprotein and Multidrug Resistance-associated Protein 1.Cancer Lett, 246：300-307，2007

24.Xu D, Lu Q, Hu X*. Downregulation of P-glycoprotein in expression in MDR brease cancer cell MCF-7/ADR by honokiol.  Cancer Lett. 243:274-280, 2006

25.Chen YD, Zheng S, Yu JK, Hu X*. Artificial neural networks analysis of surface-enhanced laser desorption/ionization mass spectra of serum protein pattern distinguishes colorectal cancer from healthy population. Clin Cancer Res 2004, 10(24):8380-5.

26.Pan Q, Fang Y, Xu Y, Zhang K, Hu X*.Down-regulation of DNA polymerases kappa, eta, iota, and zeta in human lung, stomach, and colorectal cancers. Cancer Lett. 2005,217(2):139-47.

27.Zhang K, Lu Q, Zhang Q, Hu X*.Regulation of activities of NK cells and CD4 expression in T cells by human HNP-1, -2, and -3.Biochem Biophys Res Comm. 2004, 323(2):437-44. 9

28.Hu X, Herzog C, Zimniak P, Singh SV. Differential protection against benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide-induced DNA damage in HepG2 cells stably transfected with allelic variants of pi class human glutathione S-transferase. Cancer Res. 15;59(10):2358-62,1999

29.Hu X, Xia H, Srivastava SK, Pal A, Awasthi YC, Zimniak P, Singh SV.Catalytic efficiencies of allelic variants of human glutathione S-transferase P1-1 toward carcinogenic anti-diol epoxides of benzo[c]phenanthrene and benzo[g]chrysene.Cancer Res. 8(23):5340-3,1998

30.Hu X, Pal A, Krzeminski J, Amin S, Awasthi YC, Zimniak P, Singh SV.Specificities of human glutathione S-transferase isozymes toward anti-diol epoxides of methylchrysenes.Carcinogenesis. 19(9):1685-9,1998

31.Hu X, Seidel A, Frank H, Srivastava SK, Xia H, Pal A, Zheng S, Oesch F, Singh SV. Differential enantioselectivity of murine glutathione S-transferase isoenzymes in the glutathione conjugation of trans-3,4-dihydroxy-1, 2-oxy-1,2,3,4-tetrahydrobenzo[c]phenanthrene stereoisomers.Arch Biochem Biophys. 358(1):40-8,1998

32.Hu X, Benson PJ, Srivastava SK, Xia H, Bleicher RJ, Zaren HA, Awasthi S, Awasthi YC, Singh SV. Induction of glutathione S-transferase pi as a bioassay for the evaluation of potency of inhibitors of benzo(a)pyrene-induced cancer in a murine model.Int J Cancer. 73(6):897-902,1997

33.Hu X, Singh SV.Differential catalytic efficiency and enantioselectivity of murine glutathione S-transferase isoenzymes in the glutathione conjugation of carcinogenic anti-diol epoxides of chrysene and benzo(g)chrysene.Arch Biochem Biophys. 345(2):318-24,1997

34.Hu X, Xia H, Srivastava SK, Herzog C, Awasthi YC, Ji X, Zimniak P, Singh SV. Activity of four allelic forms of glutathione S-transferase hGSTP1-1 for diol epoxides of polycyclic aromatic hydrocarbons.Biochem Biophys Res Commun. 238(2):397-402,1997

35.Hu X, Ji X, Srivastava SK, Xia H, Awasthi S, Nanduri B, Awasthi YC, Zimniak P, Singh SV. Mechanism of differential catalytic efficiency of two polymorphic forms of human glutathione S-transferase P1-1 in the glutathione conjugation of carcinogenic diol epoxide of chrysene.Arch Biochem Biophys. 345(1):32-8,1997.

36.Hu X, O'Donnell R, Srivastava SK, Xia H, Zimniak P, Nanduri B, Bleicher RJ, Awasthi S, Awasthi YC, Ji X, Singh SV.Active site architecture of polymorphic forms of human glutathione S-transferase P1-1 accounts for their enantioselectivity and disparate activity in the glutathione conjugation of 7beta,8alpha-dihydroxy-9alpha,10alpha-ox y-7,8,9,10-tetrahydrobenzo(a)pyrene.Biochem Biophys Res Commun. 235(2):424-8,1997

37.Hu X, Singh SV.Glutathione S-transferases of female A/J mouse lung and their induction by anticarcinogenic organosulfides from garlic.Arch Biochem Biophys. 340(2):279-86,1997.

38.Hu X, Srivastava SK, Xia H, Awasthi YC, Singh SV. An alpha class mouse glutathione S-transferase with exceptional catalytic efficiency in the conjugation of glutathione with 7beta, 8alpha-dihydroxy-9alpha,10alpha-oxy-7,8,9,10-tetrahydrobenzo(a)pyrene.JBiolChem. 271(51):32684-8,1996

39.Hu X, Benson PJ, Srivastava SK, Mack LM, Xia H, Gupta V, Zaren HA, Singh SV. Glutathione S-transferases of female A/J mouse liver and forestomach and their differential induction by anti-carcinogenic organosulfides from garlic.Arch Biochem Biophys. 336(2):199-214,1996

40.Pal A, Hu X, Zimniak P, Singh SV.Catalytic efficiencies of allelic variants of human glutathione S-transferase Pi in the glutathione conjugation of alpha, beta-unsaturated aldehydes.Cancer Lett. 154(1):39-43,2000

41.Pal A, Desai DH, Amin S, Srivastava SK, Hu X, Herzog C, Zimniak P, Singh SV. Location of the epoxide function determines specificity of the allelic variants of human glutathione transferase Pi toward benzo[c]chrysene diol epoxide isomers. FEBS Lett. 486(2):163-6,2000.

42.Srivastava SK, Xia H, Pal A, Hu X, Guo J, Singh SV. Potentiation of benzo[a]pyrene-induced pulmonary and forestomach tumorigenesis in mice by D,L-buthionine-S,R-sulfoximine-mediated tissue glutathione depletion. Cancer Lett. 153(1-2):35-9,2000

43.Srivastava SK, Hu X, Xia H, Awasthi S, Amin S, Singh SV. Metabolic fate of glutathione conjugate of benzo[a]pyrene-(7R,8S)-diol (9S,10R)-epoxide in human liver. Arch Biochem Biophys. 371(2):340-4,1999

44.Pal A, Seidel A, Xia H, Hu X, Srivastava SK, Oesch F, Singh SV.Specificity of murine glutathione S-transferase isozymes in the glutathione conjugation of (-)-anti- and (+)-syn-stereoisomers of benzo[g]chrysene 11,12-diol 13,14-epoxide. Carcinogenesis. 20(10):1997-2001,1999

45.Ji X, Blaszczyk J, Xiao B, O'Donnell R, Hu X, Herzog C, Singh SV, Zimniak P. Structure and function of residue 104 and water molecules in the xenobiotic substrate-binding site in human glutathione S-transferase P1-1.Biochemistry.38(32):10231-8, 1999

46.Srivastava SK, Singhal SS, Hu X, Awasthi YC, Zimniak P, Singh SV.Differential catalytic efficiency of allelic variants of human glutathione S-transferase Pi in catalyzing the glutathione conjugation of thiotepa. Arch Biochem Biophys. 366(1):89-94,1999

47.Srivastava SK, Hu X, Xia H, Pal A, Guo J, Orchard JL, Singh SV.Gender related differences in ATP-dependent transport of dinitrophenyl-glutathione conjugate across murine canalicular liver plasma membrane. FEBS Lett. 445(2-3):291-4,1999

48.Singh SV, Hu X, Srivastava SK, Singh M, Xia H, Orchard JL, Zaren HA.Mechanism of inhibition of benzo[a]pyrene-induced forestomach cancer in mice by dietary curcumin. Carcinogenesis. 19(8):1357-60,1998

49.Singh SV, Benson PJ, Hu X, Pal A, Xia H, Srivastava SK, Awasthi S, Zaren HA, Orchard JL, Awasthi YC. Gender-related differences in susceptibility of A/J mouse to benzo[a]pyrene-induced pulmonary and forestomach tumorigenesis. Cancer Lett. 128(2):197-204,1998

50.Srivastava SK, Hu X, Xia H, Bleicher RJ, Zaren HA, Orchard JL, Awasthi S, Singh SV. ATP-dependent transport of glutathione conjugate of 7beta, 8alpha-dihydroxy-9alpha,10alpha-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene in murine hepatic canalicular plasma membrane vesicles.Biochem J  332 (3):799-805,1998

51.Xia H, Pan SS, Hu X, Srivastava SK, Pal A, Singh SV. Cloning, expression, and biochemical characterization of a functionally novel alpha class glutathione S-transferase with exceptional activity in the glutathione conjugation of (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo(a)pyrene.Arch Biochem Biophys. 353(2):337-48,1998

52.Singh SV, Pan SS, Srivastava SK, Xia H, Hu X, Zaren HA, Orchard JL.Differential induction of NAD(P)H:quinone oxidoreductase by anti-carcinogenic organosulfides from garlic.Biochem Biophys Res Commun. 244(3):917-20,1998

53.Srivastava SK, Hu X, Xia H, Zaren HA, Chatterjee ML, Agarwal R, Singh SV.Mechanism of differential efficacy of garlic organosulfides in preventing benzo(a)pyrene-induced cancer in mice.Cancer Lett. 118(1):61-7,1997.

54.Xia H, Bleicher RJ, Hu X, Srivastava SK, Gupta V, Zaren HA, Singh SV.Characterization of a BMS-181174-resistant human bladder cancer cell line.Br J Cancer. 76(4):461-6,1997

55.Singh SV, Mohan RR, Agarwal R, Benson PJ, Hu X, Rudy MA, Xia H, Katoh A, Srivastava SK, Mukhtar H, Gupta V, Zaren HA.Novel anti-carcinogenic activity of an organosulfide from garlic: inhibition of H-RAS oncogene transformed tumor growth in vivo by diallyl disulfide is associated with inhibition of p21H-ras processing.Biochem Biophys Res Commun. 225(2):660-5,1996

56.Hu X, Yang H, Pan QR, Zheng S. Does P-glycoprotein play a pivotal role in the drug resistance of an MDR variant, K562/Dox? Chemotherapy. 41(4):296-305,1995.

57.Hu X, Arnold WM, Zimmermann U. Alterations in the electrical properties of T and B lymphocyte membranes induced by mitogenic stimulation. Activation monitored by electro-rotation of single cells. Biochim Biophys Acta. 1021(2):191-200,1990

招生信息

招收肿瘤学研究方向学术硕士及博士。