研究生教育

    其重点聚焦医工交叉，在单分子水平，研究力学-化学耦联调控重要膜蛋白（T细胞受体等）的肿瘤抗原识别和激活的分子机制，并利用单细胞技术开展肿瘤早期筛查及肿瘤等重大疾病免疫图谱的动态演变等。相关学术成果已作为第一作者或者通讯作者在多个国际顶级及专业期刊上发表论文,包括Cell、Cell Res.(2)、Mol.Cell、EMBO J.、Nano Letter、eLife、JCB等。作为共同作者，还在Nat. Immune、Immunity、PNAS等其他高水平杂志上发表多篇学术论文。近期，其主攻单分子动态技术研究新冠病毒突刺(Spike) 蛋白入侵宿主的动态识别ACE2和激活构象变化的分子机制，探索新冠病毒入侵宿主的机理，希望能够对研发新型的新冠抗体及疫苗提供新的思路。中国生物医学工程生物力学专业委员会会委员，中国基质生物学会委员，中国抗癌协会肿瘤精准治疗委员会委员，中国细胞生物学学会专业分会委员，中国生物物理学会膜生物学专业分会理事。

研究领域

单分子及单细胞技术 | 力生物学与生物力学 | 蛋白质动态结构与功能 | 分子免疫学及免疫工程 | 分子神经生物学 | 系统生物学

论文：

1.        Yinxuan Lei*, Panyu Fei*, Bin Song, Wenjia Shi, Cheng Luo, Dahai Luo, Dan Li#, Wei Chen#, Jie Zheng#, A loosened gating mechanism of RIG-I leads to autoimmune disorders, Nucleic Acids Research, 2022;

2.        Jiawei Shi, Junwei Liu, Xiaoxuan Tu, Bin Li, Zhou Tong, Tian Wang, Yi Zheng, Hongyu Shi, Xun Zeng#, Wei Chen#, Weiwei Yin# and Weijia Fang#, Single-Cell immune signature for detecting early-stage HCC and early assessing anti-PD-1 immunotherapy efficacy, Journal of Immunotherapy for Cancer, 2022

3.        Juan Fan*, Jiawei Shi*, Yong Zhang*, Junwei Liu, Chenyi An, Huaying Zhu, Peng Wu, Wei Hu, Rui Qin, Danmei Yao, Xin Shou, Yibing Xu, Zhou Tong, Xue Wen, Jianpo Xu, Jin Zhang, Weijia Fang, Jizhong Lou#, Weiwei Yin#, Wei Chen#, NKG2D discriminates diverse ligands through selectively mechano‐regulated ligand conformational changes, EMBO J. , 2021

4.        Panyu Fei, Haibo Ding, Yu Duan, Xinyi Wang, Wei Hu, Peng Wu, Mengxiao Wei, Zhengchun Peng#, Zhongze Gu#, Wei Chen#, Utility of TPP-manufactured biophysical restrictions to probe multiscale cellular dynamics, Bio-Design and Manufacturing, 2021;

5.        An Chenyi, Chen Wei, Multiplexed single-molecule force spectroscopy for dissecting biophysical regulation of membrane receptors functions on live cells, Biophysics Reports, 2021;

6.        Yan Shi#, Erdinc Sezgin#, Wei Chen#,The Role of Biomembranes and Biophysics in Immune Cell Signaling, Frontiers in Immunonlogy, 2021;

7.        Wei Hu*, Yong Zhang*, Panyu Fei*, Tongtong Zhang*, Danmei Yao*, Yufei Gao, Jia Liu, Hui Chen, Qiao Lu, Tenny Mudianto, Xinrui Zhang, Chuxuan Xiao, Yang Ye, Qiming Sun, Jing Zhang, Qi Xie, Pei-Hui Wang, Jun Wang#, Zhenhai Li#, Jizhong Lou#, Wei Chen#,Mechanical activation of spike fosters SARS-CoV-2 viral infection, Cell Research, 2021

8.        Junwei Liu, Lulu Liu, Saisi Qu, Tongtong Zhang, Danyang Wang, Qinghua Ji, Tian Wang, Hongyu Shi, Kaichen Song, Weijia Fang, Wei Chen#, Weiwei Yin#, GdClean: removal of Gadolinium contamination in mass cytometry data, Bioinformatics, 2021

9.        Tongtong Zhang*, Wei Hu*, Wei Chen, Plasma Membrane Integrates Biophysical and Biochemical Regulation to Trigger Immune Receptor Functions, Frontiers in Immunology 12, 53, 2021；

10.     J Liu, S Qu, T Zhang, Y Gao, H Shi, K Song, W Chen#, W Yin#, Applications of single-cell omics in tumor immunology, Frontiers in Immunology ,12, 2021;

11.     Hui Chen, Yang Cui, Xuling Han, Wei Hu, Min Sun, Yong Zhang, Pei-Hui Wang, Guangtao Song, Wei Chen#, Jizhong Lou#, 'Liquid–liquid phase separation by SARS-CoV-2 nucleocapsid protein and RNA',Cell Research, 2020,

12.     Chenyi An*, Wei Hu*, Jie Gao, Bingfeng Ju, Peyman Obeidy, Yunduo Charles Zhao, Xiaoxuan Tu, Weijia Fang#, Lining Arnold Ju#, and Wei Chen#, 'Ultra-stable biomembrane force probe for accurately determining slow dissociation kinetics of PD-1 blockade antibodies on single living cells', Nano Letter, 2020

13.     Wei Hu*, Yong Zhang*, Xiaolin Sun*, Tongtong Zhang, Liling Xu, Hengyi Xie, Zhanguo Li, Wanli Liu#, Jizhong Lou#, Wei Chen#，'FcγRIIB-I232T polymorphic change allosterically suppresses ligand binding', eLife, 2019

14.     Cheng Zhu#, Wei Chen, Jizhong Lou, William Rittase, Kaitao Li, Mechanosensing through immunoreceptors, Nat. Immun., 2019 20,1269-1278.;

15.     Peng Wu*, Tongtong Zhang*, Baoyu Liu*, Panyu Fei*, Lei Cui*, Rui Qin, Huaying Zhu, Danmei Yao, Ryan J Martinez, Wei Hu, Chenyi An, Yong Zhang, Junwei Liu, Jiawei Shi, Juan Fan, Weiwei Yin, Jie Sun, Chun Zhou, Xun Zeng, Chenqi Xu, Jianan Wang, Brian Evavold, Cheng Zhu, Wei Chen#$, Jizhong Lou#, 'Mechano-regulation of Peptide-MHC Class I Conformations Determines TCR Antigen Recognition', Molecular Cell, 2019 ($Lead contact)

16.     Zhengpeng Wan, Chenguang Xu, Xiangjun Chen, Hengyi Xie, Zongyu Li, Jing Wang, Xingyu Ji, Haodong Chen, Qinghua Ji, Samina Shaheen, Yang Xu, Fei Wang, Zhuo Tang, Ji-Shen Zheng, Wei Chen, Jizhong Lou, Wanli Liu,'PI (4, 5) P2 determines the threshold of mechanical force–induced B cell activation',J Cell Biol,2018

17.     Chenyi An, Panyu Fei, Wei Chen, 'Power of one: Investigating Protein Dynamic Functions using Single-Molecule Force Spectroscopy', Chinese Journal of Biochemistry and Molecular Biology, 2017, Vol.33,10,965-978

18.     Samina Shaheen, Zhengpeng Wan, Zongyu Li, Alicia Chau, Xinxin Li, Shaosen Zhang, Yang Liu, Junyang Yi, Yingyue Zeng, Jing Wang, Xiangjun Chen, Liling Xu, Wei Chen, Fei Wang, Yun Lu, Wenjie Zheng, Yan Shi, Xiaolin Sun, Zhanguo Li, Chunyang Xiong, Wanli Liu, 'Substrate stiffness governs the initiation of B cell activation by the concerted signaling of PKCβ and focal adhesion kinase',Elife,2017,e23060

19.     Lining Ju, Yunfeng Chen, Muaz Nik Rushdi, Wei Chen, Cheng Zhu, 'Two-dimensional analysis of cross-junctional molecular interaction by force probes', The Immune Synapse (Book Chapter), 2017, 231-258

20.     Liu B.*, Chen W.*, Natarajan K., Li Z., Margulies D., Zhu C., 'The cellular environment regulates in situ kinetics of T-cell receptor interaction with peptide major histocompatibility complex”. European Journal of Immunology, 2015; (*co-first author)

21.     Chen Y.*,  Liu B.*, Ju L.*, Hong J.*, Ji Q., Chen W.#, Zhu C.# ,Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-Ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell , Journal of Visualized Experiments; ( # co-corresponding author)

22.     Hu W., An C., Chen W.#, “Molecular Mechanoneurobiology: An Emerging Angle to Explore Neural Synaptic Functions”.  BioMed Research International, 2015; (# corresponding author)

23.     Liu, B., Chen, W., Zhu, C. , Molecular Force Spectroscopy on Cells. Annu. Rev. Phys. Chem., 2015;

24.     Liu B. *, Chen W. *, Evavold B., Zhu C., “Antigen-specific TCR–pMHC catch triggers T-cell signaling by rapidly accumulating successive bond lifetimes prolonged by optimal force”, Cell, 2014, 157: 357–368. (*co-first author)

25.     Choi Y. *, Duke-Cohan J.*, Chen W., Liu B., Rossy J., Gui J., Gaus K., Zhu C. and Reinherz E., “PlexinD1 regulates dynamic adhesion in beta1 integrin nanoclusters via control of catch bonds”, PNAS, 2013, 111: 379–384.  (*co-first author)

26.     Chen W., Zhu C., “ Mechanical regulation on T-cell functions”, invited review for Immunological Review, 2013, 256: 160–176.

27.     Chen W., Lou J., Evans, E., Zhu C., “Observing force-regulated conformational changes and ligand dissociation from a single integrin on cells”, J Cell Biol, 2012, 199: 497–512. (Commented by the Journal of cell Biology in the same issue)

28.     Adams J., Narayanan S., Liu B., Birnbaum M., Kruse A., Bowerman N., Chen W., Levin A., Connolly J., Zhu C., Kranz D., Garcia C., “T cell receptor signaling is limited by docking geometry to peptide major histocompatibility complex”, Immunity, 35:681-693, 2011.

29.     Xiang X. *, Lee C. *, Li T., Chen W., Zhu C., Lou J., “Structural basis and kinetics of force-induced conformational changes of an alpha-A domain-containing integrin ” Plos One, 6(11):e27946, 2011.  (*co-first author)

30.     Sarangapni K. *, Qian J. *, Chen W., Zarnitsyna V., McEver R., Zhu C., “Regulation of catch bonds by rate of force application” J. of Biological Chemistry, 286(37):32749, 2011. (*co-first author)

31.     Chen W., Lou J., Zhu C., “Forcing switch from short- to intermediate- and long-lived states of the alphaA domain generates LFA-1/ICAM-1 catch bonds”, Journal of Biological Chemistry, 285(46):35957-35978, 2010.

32.     Wayman A.M., Chen W., McEver R.P., Zhu C., “Triphasic force dependence of E-selectin/ligand dissociation governs cell Rolling under flow”, Biophysical Journal, 99:1166-1174, 2010.

33.     Chen W., Zarnitsyna I.V., Sarangapani K.K., Huang J., and Zhu C., “Measuring receptor-ligand binding kinetics on cell surfaces: from adhesion frequency to thermal fluctuation methods”, Cellular and Molecular Bioengineering, 4: 276–288, 2008.

34.     Chen W., Evans E., McEver R.P., and Zhu C., “Monitoring receptor-ligand interactions between surfaces by thermal fluctuations”, Biophysical Journal, 94: 694-701, 2007.

35.     Wang C., Chen W., Zhu S. “Novel central meter reading system by telephone”, Electrical Automation, 25(4): 44-45, 2004 (In Chinese).

36.     Lou J. *, Yago T. *, Klopocki A. G. *, Metha P., Chen W., Zarnitsyna V. I., Bovin N. V., Zhu C., and McEver R. P., “Flow-enhanced adhesion regulated by a selectin interdomain hinge”, J Cell Biol.  174:1107-1117, 2006. (*co-first author)

37.     Chen W., Mei D., Zhu S., “A research on Isolated Vibrations Control System of Micro-manufacturing Platform”. The Proceeding of the American Control Conference, 2002.

38.     Chen W., Zhu S., “Research of low power consumption intelligent heat meter”, The Journal of Mechanical and Electrical Engineering, 18(5), 2002 (In Chinese).

39.     Chen W., He W., Pilot Study of Dynamic Parameters of Vibration Isolation Control System of Micro-manufacturing Platform. Proceedings of the 2nd Academic Forum of Micro-System between Taiwan Strait, 2001.10, Hangzhou, China, 2001 (In Chinese).

实验室正在积极招收博士后、博士研究生！

热诚欢迎各个学科背景（包括生物学、工程、计算机、物理、化学、生物化学、分子生物学、结构生物学、免疫学、生物技术、生物工程、生物信息学等）的本科、硕士及博士加入！